Our Team
Jean-Philippe Galaud
Professor UPS - Group Leader
jean-philippe.galaud@univ-tlse3.fr
Didier Aldon
Assistant Professor UPS
didier.aldon@univ-tlse3.fr
Malick Mbengue
Assistant Professor UPS
malick.mbengue@univ-tlse3.fr
Artemis Perraki
Researcher, CNRS
artemis.perraki@univ-tlse3.fr
Eugénie Robe
Assistant Engineer UPS
eugenie.robe@univ-tlse3.fr
Sabine Grat-Simeone
Technician UPS
sabine.grat-simeone@univ-tlse3.fr
Sarah Carpenter
PhD student
sarah.carpentier@univ-tlse3.fr
Eve Teyssier
PhD Student
eve.teyssier@univ-tlse3.fr
Rafail Gkritzas
Engineer
rafail.gkritzas@univ-tlse3.fr
Vasiliki Katidou
PhD student
vasiliki.katidou@univ-tlse3.fr
Mathilde Ouradou
Master 2 student
mathilde.ouradou@univ-tlse3.fr
Nguyen Thu Hong
Master 2 student
thuhong0201@gmail.com
Former members
Amandine Lucchin (PhD student)
Léa Casagrande (Engineer ANR)
Julie Mazard (PhD Student)
Christian Mazars (Research Director CNRS)
Valérie Cotelle (Lecturer/Researcher UPS)
Patrice Thuleau (Researcher CNRS)
Manon Perez (PhD Student)
Julia Audabram (Assistant Engineer ANR)
Sarah Pignoly (Engineer ANR)
Ambroise TESTARD (PhD Student)
Mélanie ORMANCEY (PhD Student)
Xiaoyang ZHU (PhD Student)
Christian BRIERE (Researcher CNRS)
Martin GACHENOT (Intern)
Mouna GHORBEL (PhD Student)
Yann AUBERT (PhD Student)
Martine CHARPENTEAU (Engineer UPS)
Cecilia CHEVAL (PhD Student)
Daniel Da SILVA (PhD Student)
Annick GRAZIANA (Professorr UPS)
Christophe LACHAUD (PhD Student)
Louis-Jérôme LEBA (PhD Student)
Alexandre PEROCHON (PhD Student)
Elsa PRIGENT (PhD Student)
Raoul RANJEVA (Research Director CNRS)
Tou Cheu XIONG (Researcher INRA)
In plants, one of the first responses observed following the perception of biotic and/or abiotic environmental stimuli is a modulation of free calcium concentration in the cell. These calcium variations can take many forms, and are an integral part of the cell signalling that leads to specific responses. For many years, the team has been exploring calcium-dependent signaling pathways (monitoring calcium variations and decoding processes by a family of plant-specific calmodulin-like proteins, Calmodulin like- CMLs) and their targets. Our work has now been extended to link calcium signaling with perception systems, via receptors localized at the plasma membrane.
The team aims to better understand the signaling mechanisms governing plant-microorganism interactions (immunity and symbiosis), plant development (ie root architecture and plant reproductive development) and how these are influenced by environmental changes (ie temperature). New models, complementary to Arabidopsis, have been introduced into the team to facilitate this work (Marchantia) and transfer the data acquired to plants of interest (tomato).
Our scientific objectives are:
1) to identify and characterise novel plant receptor complexes governing plant development and plant-microorganism interactions
2) to monitor the receptor mediated cytosolic Ca2+ variations (signatures) that control plant development and response to stress at both cellular and organ level
3) to decipher the role of calcium sensors (calmodulin-type proteins and their targets) in plant physiology and plant immune responses, taking into consideration the effect of temperature
- Analysis of calcium variations
- Perception events in Marchantia regulating arbuscular mycorrhiza formation
- Investigation of the plasma membrane receptor associated signalling processes that control early pollen development
- Calcium sensors and environmental stresses
Analysis of calcium variations
Contacts : Sabine Grat-Simeone, Eugénie Robe, Artemis Perraki, Malick Mbengue, Didier Aldon, Jean-Philippe Galaud
Very often, variation in calcium concentration in the cytosol, or other subcellular compartments such as the nucleus, mitochondria or chloroplasts is part of plant cell responses to stimuli. This subcellular localization of the calcium signal combined with other parameters such as intensity, duration, shape or frequency define the so-called “calcium signature”.
By using the jellyfish-derived calcium probe aequorin, we are able to follow real-time calcium variations in the cytosol and the nucleus. In presence of the chromophore coelentarazine, apo-aequorin is capable of binding 3 calcium ions. This provokes a conformational change followed by an intramolecular oxidation that emits blue light at 468nm. The light emission is proportional to the calcium concentration and can be captured by a sensitive PMT-based luminometer.
Using this approach, our group has demonstrated:
- The contribution of nuclei in defining a specific calcium signature
- The autonomy of nuclei in producing a calcium signal when challenged with external stimuli
- The possibility for nuclei to control, independently or via the cytosol, responses implicating calcium signalling.
The recent development of fluorescent reporters of cytoplasmic Ca2+ concentration allows the quantification of cytosolic calcium transients in real time and in specific cell compartments and cell types, or in entire organs and plants. The team is currently exploring the highly sensitive calcium indicator R-GECO and GCAMP to discriminate and quantify the stimulus-dependent directional propagation of Ca2+ in immunity, following heat stress and during development.
Collaborations :
Le Ru A., Toulouse, France
De Carvalho-Niebel F., Toulouse France
Navarro L, Paris, France
Marc Knight, UK
Perception events in Marchantia regulating arbuscular mycorrhiza formation
Contacts : Sabine Grat-Simeone, Eugénie Robe, Didier Aldon, Artemis Perraki, Jean-Philippe Galaud, Malick Mbengue
To acquire water and nutrients, most land plants form the arbuscular mycorrhizal symbiosis (AMS) with soil-borne Glomeromycota fungi in which the fungus extracts nutrients from the soil in exchange of photosynthates. This exchange occurs within the cells of the host tissues at a tree-shaped interface formed by both partners and called the arbuscule. This symbiosis evolved around half a billion years ago and this evolutionary innovation was key in driving land colonization by plants. AMS establishment depends on signals exchanges between partners and on the activation of a plant-signalling pathway where calcium variations both in the cytosol and in the nucleus are crucial. While receptors from the LysM-RLKs family are the best candidates for early perception of the fungal partner, study of this family of receptors in flowering plants remains challenging due to genetic redundancy. Therefore, the identity and interplay of plant receptors complexes regulating the very first steps of this molecular dialogue are to date elusive. Importantly, AMS is not restricted to vascular plants and occurs as well in bryophytes such as the liverwort Marchantia paleacea. To our advantage, Marchantia did not undergo genome wide duplications and it harbours a gametophyte dominant lifestyle. These two points combined very often simplify genetic studies when compared to angiosperms. By using a combination of molecular biology, genetics and calcium monitoring as a proxy for signalling pathway activation, this research axis focuses on deciphering the early signalling events at the base of the most widespread symbiosis on Earth.
Investigation of the plasma membrane receptor associated signalling processes that control early pollen development
Contacts : Sabine Grat-Simeone, Eugénie Robe, Didier Aldon, Jean-Philippe Galaud, Artemis Perraki
This research axis aims to improve our understanding of the plasma membrane associated receptors regulating the development of plant male reproductive lineage, their mechanism of action and their interplay with calcium signalling.
In flowering plants, plant male reproductive lineage (germline) formation is critical for pollen production, the success of the sexual reproduction and grain yield. This requires the formation of distinct sub-epidermal cell layers in the anther, such as the tapetum which is the cell layer that surrounds the reproductive cells that will undergo meiosis to generate haploid microspores, which will further develop to pollen grains. Defects in either tapetum or meiocytes often lead to male sterility.
In plants, plasma membrane- associated receptors such as leucine riche receptor kinases (LRR-RKs) perceive extracellular signals and regulate plant development and response to stress. Receptor signalling activation is often associated with the generation of Ca2+ signals, important for signal amplification and relay. For instance, there is accumulating evidence that Calmodulin-like proteins (CMLs) may directly feedback to and regulate LRR-RK complex activation.
Our research goal is to improve our understanding of the LRR-RK associated processes controlling early pollen development. Specifically, we aim to: 1) identify novel receptor complexes acting in tapetum and meiocytes and characterise their role and 2) to present the first links between receptor signalling activation, the generation of Ca2+ signals and its integration by calcium sensors (of the CML family) during early pollen development.
Because tapetum formation is a developmental stage particularly vulnerable to temperature elevations, with severe consequences for male fertility, pollen production and thus yield loss, we also aim: 3) to explore potential roles of LRR-RK signalling and Ca2+ sensors (CMLs) in regulating thermal sensitivity in the tapetum and 4) to provide insights on the tapetum specific transcriptional landscape following acute heat stress in Arabidopsis.
Acquired funding:
– ANR JCJC project “SIGNAL”
– TULIP LabEx New Frontiers project “RE-HEAT”
– EUR TULIP-GS and LabEx TULIP PhD Position Grant
Calcium sensors and environmental stresses
Contacts : Sabine Grat-Simeone, Eugénie Robe, Artemis Perraki, Malick Mbengue, Didier Aldon, Jean-Philippe Galaud
The third axis of research aims to better understand how these calcium variations are decoded, amplified and relayed at the cellular level. Calcium decoding is carried out by relay proteins, such as calmodulin (CaM), which regulate the activity of target proteins following binding to Ca2+. Using molecular and genetic approaches, our group revealed the importance of calmodulins and related proteins (calmodulin like or CMLs) and some of their plant-specific targets in stress responses.
For several years, we have mainly focused on the importance of calcium signaling in response to biotic stimuli and more recently, we have extended our work to other Arabidopsis and Tomato CMLs to evaluate their contribution in the processes of development and physiology of plants in relation to biotic and abiotic constraints. This work is part of a more general objective which is to better understand the defense responses of plants to pathogens depending on changes in environmental conditions such as slight variations in temperature. Indeed, a slight increase in temperature (3 to 5°C) significantly affects the resistance of plants to bacteria, fungi, viruses and insects, but the data remains fragmentary. Although work has identified regulators of these stresses applied separately, our knowledge of the mechanisms allowing plants to respond simultaneously to multiple biotic and abiotic attacks remains to be explored.
As CMLs and their targets are at the crossroads of biotic and abiotic signaling pathways and developmental processes, our objectives are to decipher and understand their contribution in different processes using different plant models to respond to both (i) fundamental questions raised (Arabidopsis) and (ii) to transfer the results acquired to plants of agronomic interest (tomato). This project also meets the objectives of Labex “TULIP” by revealing the importance of calcium signaling and the effects of the environment on plant development and in plant-microorganism interactions.
Collaborations :
– Richard Berthomé, Toulouse, France
– Xiaoyang Zhu, Guangzhou, South China Agricultural University, China
– Wayne Snedden, Kingston, Canada
– Axel Mithöfer, Iena, Germany
– Marc Knight, UK
– Réseau « Résistance des plantes » managed by JB Morel and L Deslandes
– Membre du Groupement d’Intérêt Scientifique « Biotechnologies Vertes » (GIS BV)
– Réseau Impact of ENvironment on plant immunity and pathogen VIrulencE (ENVIE) managed by R Berthomé and M Fagard
– PhD position grant from EUR TULIP-GS and Région Occitanie
2021
Plant J. 2021 Ethylene signaling modulates tomato pollen tube growth through modifications of cell wall remodeling and calcium gradient. Aug;107(3):893-908. doi: 10.1111/tpj.15353. Epub 2021 Jun 20.
The Same against Many: AtCML8, a Ca2+ Sensor Acting as a Positive Regulator of Defense Responses against Several Plant Pathogens. Int J Mol Sci. 2021 Sep 28;22(19):10469. doi: 10.3390/ijms221910469.
Xiong T, Tan Q, Li S, Mazars C, Galaud JP, Zhu X., 2021. Interactions between calcium and ABA signaling pathways in the regulation of fruit ripening. J Plant Physiol. 2021 doi: 10.1016/j.jplph.2020.153309.
Ca2+-Dependent Protein Kinase 6 Enhances KAT2 Shaker Channel Activity in Arabidopsis thaliana. Int J Mol Sci. 2021 Feb 5;22(4):1596. doi: 10.3390/ijms22041596.
Kusch S, Larrouy J, Ibrahim HMM, Mounichetty S, Gasset N, Navaud O, Mbengue M, Zanchetta C, Lopez-Roques C, Donnadieu C, Godiard L, Raffaele S., 2021. Transcriptional response to host chemical cues underpins the expansion of host range in a fungal plant pathogen lineage. The ISME Journal 16, 138–148
2022
Carpentier S, Aldon D, Berthomé R, Galaud JP, 2022. Is there a specific calcium signal out there to decode combined biotic stress and temperature elevation? Front Plant Sci. doi: 10.3389/fpls.2022.1004406..
2023
Ferrer-Orgaz S, Tiwari M, Isidra-Arellano MC, Pozas-Rodriguez EA, Vernié T, Rich MK, Mbengue M, Formey D, Delaux PM, Ané JM, Valdés-López O., 2023. Early Phosphorylated Protein 1 is required to activate the early rhizobial infection program. New Phytologist 241, 962–968.
Liu C, Mentzelopoulou A, Papagavriil F, Ramachandran P, Perraki A, Claus L, Barg S, Dörmann P, Jaillais Y, Johnen P, Russinova E, Gizeli E, Schaaf G, Moschou PN., 2023. SEC14-like condensate phase transitions at plasma membranes regulate root growth in Arabidopsis. PLoS Biol. 18;21(9):e3002305. doi: 10.1371/journal.pbio.3002305.
Goto, Yukihisa, Yasuhiro Kadota, Malick Mbengue, Jennifer D Lewis, Hidenori Matsui, Noriko Maki, Jan Sklenar, et al. « The leucine-rich repeat receptor kinase QSK1 is a novel regulator of PRR-RBOHD complex and is employed by the bacterial effector HopF2P to modulate plant immunity », (2023). https://doi.org/10.1101/2023.12.13.571443. Preprint.
2024
Zhu Q., Tan Q., Gao Q., Zheng S., Chen W., Galaud J.P., Li X., Zhu X., 2024. Calmodulin-like protein CML15 interacts with PP2C46/65 to regulate papaya fruit ripening via integrating calcium, ABA and ethylene signals. Plant Biotechnology Journal. DOI: 10.1111/pbi.14297.
Teyssier, E., Grat, S., Rich, M., Delaux, P.-M. & Mbengue, M. LysM-RLK plays an ancestral symbiotic function in plants. (2024) doi:10.1101/2024.01.16.575821. Preprint.
Vernié, T., Rich M., Pellen T., Teyssier E., Garrigues V., Chauderon L., Medioni L., van Beveren F., Libourel C., Keller J., Girou C., Lefort C., Le Ru A., Reinhardt D., Kodama K., Shimazaki S., Morel P., Kyozuka J., Mbengue M., Vandenbussche M., Delaux P.M. Conservation of symbiotic signalling across 450 million years of plant evolution. (2024) doi:10.1101/2024.01.16.575147. Preprint.
2020
Barbacci A, Navaud O, Mbengue M, Barascud M, Godiard L, Khafif M, Lacaze A, Raffaele S. (2020) Rapid identification of an Arabidopsis NLR gene as a candidate conferring susceptibility to Sclerotinia sclerotiorum using time-resolved automated phenotyping. Plant J. 2020 Jul;103(2):903-917. doi: 10.1111/tpj.14747.
Sucher J, Mbengue M, Dresen A, Barascud M, Didelon M, Barbacci A, Raffaele S. (2020) Phylotranscriptomics of the Pentapetalae Reveals Frequent Regulatory Variation in Plant Local Responses to the Fungal Pathogen Sclerotinia sclerotiorum. Plant Cell. 2020 Jun;32(6):1820-1844. doi: 10.1105/tpc.19.00806.
Derbyshire M, Mbengue M, Barascud M, Navaud O, Raffaele S. (2020) Small RNAs from the plant pathogenic fungus Sclerotinia sclerotiorum highlight host candidate genes associated with quantitative disease resistance. Mol Plant Pathol. 2019 Sep;20(9):1279-1297. doi: 10.1111/mpp.12841.
2019
Dell’Aglio E, Giustini C, Kraut A, Couté Y, Costa A, Decros G, Gibon Y, Mazars C, Matringe M, Finazzi G, Curien G. (2019) Identification of the Arabidopsis Calmodulin-Dependent NAD+ Kinase That Sustains the Elicitor-Induced Oxidative Burst. Plant Physiol. 181(4):1449-1458.
Bourque S, Lindermayr C, Mazars C., (2019) Post-translational Modifications in Plant Nuclear Signaling: Novel Insights Into Responses to Environmental Changes. Front Plant Sci. 2019 Feb 11;10:104. doi: 10.3389/fpls.2019.00104
Peyraud R, Mbengue M, Barbacci A, Raffaele S (2019) Intercellular cooperation in a fungal plant pathogenfacilitates host colonization. Proc Natl Acad Sci USA 116 (8) 3193-3201
2018
Aldon D, Mbengue M, Mazars C, Galaud JP (2018) Calcium Signalling in Plant Biotic Interactions. Int J Mol Sci. [http://www.mdpi.com/1422-0067/19/3/665]
Favre L., Ortalo-Magné A., Pichereaux C., Gargaros A., Burlet-Schiltz O., Cotelle V. and Culioli G. (2018). Metabolome and proteome changes between biofilm and planktonic phenotypes of the marine bacterium Pseudoalteromonas lipolytica TC8. Biofouling. 34 : 132-148.
Heyer M, Scholz SS, Voigt D, Reichelt M, Aldon D, Oelmüller R, Boland W, Mithöfer A., 2018. Herbivory-responsive calmodulin-like protein CML9 does not guide jasmonate-mediated defenses in Arabidopsis thaliana. PLoS One. 16;13(5):e0197633. [https://pubmed.ncbi.nlm.nih.gov/29768484/]
2017
Zhu X, Perez M, Didier Aldon D & JP Galaud (2017) Respective contribution of CML8 and CML9, two arabidopsis calmodulin-like proteins, to plant stress responses.[http://www.tandfonline.com/doi/full…]
Ghorbel M., Cotelle V., Ebel C., Zaidi I., Ormancey M., Galaud J.P., Hanin M. (2017) Regulation of the wheat MAP kinase phosphatase 1 by 14-3-3 proteins. [http://www.sciencedirect.com/scienc…] 257, 37–47.
Zhu X, Robe E, Jomat L, Aldon A, Mazars C, Galaud JP (2017) Calmodulin-like 8 contributes to plant immunity.[https://academic.oup.com/pcp/articl…] 58(2):307-319
Ormancey M, Thuleau P, Mazars C, Cotelle V (2017) CDPKs and 14-3-3 Proteins : Emerging Duo in Signaling. Trends Plant Sci. S1360-1385(16)30190-X.
Badet T*, Peyraud R*, Mbengue M*, Navaud O, Derbyshire M, Oliver RP, Barbacci A, Raffaele S (2017) Codon optimization underpins generalist parasitism in fungi. Elife
Badet T, Voisin D, Mbengue M, Barascud M, Sucher J, Sadon P, Balague C, Roby D, Raffaele S (2017) Parallel evolution of the POQR prolyl oligo peptidase gene conferring plant quantitative disease resistance. PLoS Genet 13: e1007143
Bucherl CA, Jarsch IK, Schudoma C, Segonzac C, Mbengue M, Robatzek S, MacLean D, Ott T, Zipfel C (2017) Plant immune and growth receptors share common signalling components but localise to distinct plasma membrane nanodomains. Elife 6
Derbyshire M, Denton-Giles M, Hegedus D, Seifbarghy S, Rollins J, van Kan J, Seidl MF, Faino L, Mbengue M, Navaud O, Raffaele S, Hammond-Kosack K, Heard S, Oliver R (2017) The complete genome sequence of the phytopathogenic fungus Sclerotinia sclerotiorum reveals insights into the genome architecture of broad host range pathogens. Genome Biol Evol 9: 593-618
2016
Testard A, Da Silva D, Ormancey M, Pichereaux C, Pouzet C, Jauneau A, Grat S, Robe E, Brière C, Cotelle V, Mazars C, Thuleau P (2016) Calcium- and Nitric Oxide-Dependent Nuclear Accumulation of Cytosolic Glyceraldehyde-3-Phosphate Dehydrogenase in Response to Long Chain Bases in Tobacco BY-2 Cells. Plant Cell Physiol 57 (10) :2221-2231
Ranty B, Aldon D, Cotelle V, Galaud JP, Thuleau P, Mazars C (2016) Calcium sensors as key hubs in plant responses to biotic and abiotic stresses Front. Plant Sci 7:327
Cotelle V, Leonhardt N (2016) 14-3-3 proteins in guard cell signaling. Front.Plant Sci 6:12
Mazars C ; Ranty B ; Aldon D ; Oelmüller R ; Galaud J-P ; Mithöfer A (2016) CMLs control host-plant interactions.Endocytobiosis and Cell Research 27:13-19
Mbengue M*, Bourdais G*, Gervasi F, Beck M, Zhou J, Spallek T, Bartels S, Boller T, Ueda T, Kuhn H, Robatzek S (2016) Clathrin-dependent endocytosis is required for immunity mediated by pattern recognition receptor kinases. Proc Natl Acad Sci U S A 113: 11034-11039
Mbengue M, Navaud O, Peyraud R, Barascud M, Badet T, Vincent R, Barbacci A, Raffaele S (2016) Emerging Trends in Molecular Interactions between Plants and the Broad Host Range Fungal Pathogens Botrytis cinerea and Sclerotinia sclerotiorum. Front Plant Sci 7: 422
Mithoe SC, Ludwig C, Pel MJ, Cucinotta M, Casartelli A, Mbengue M, Sklenar J, Derbyshire P, Robatzek S, Pieterse CM, Aebersold R, Menke FL (2016) Attenuation of pattern recognition receptor signaling is mediated by a MAP kinase kinase kinase. EMBO Rep 17: 441-454
Postma J, Liebrand TW, Bi G, Evrard A, Bye RR, Mbengue M, Kuhn H, Joosten MH, Robatzek S (2016) Avr4 promotes Cf-4 receptor-like protein association with the BAK1/SERK3 receptor-like kinase to initiate receptor endocytosis and plant immunity. New Phytol 210: 627-642
Vernie T, Camut S, Camps C, Rembliere C, de Carvalho-Niebel F, Mbengue M, Timmers T, Gasciolli V, Thompson R, le Signor C, Lefebvre B, Cullimore J, Herve C (2016) PUB1 Interacts with the Receptor Kinase DMI2 and Negatively Regulates Rhizobial and Arbuscular Mycorrhizal Symbioses through Its Ubiquitination Activity in Medicago truncatula. Plant Physiol 170: 2312-2324
2015
Ghorbel M, Zaidi I, Robe E, Ranty B, Mazars C, Galaud JP, Hanin M (2015) The activity of the wheat MAP kinase phosphatase 1 is regulated by manganese and by calmodulin. Biochimie 108 : 13-19
Zhu X, Dunand C, Snedden W, Galaud JP (2015) CaM and CML emergence in the green lineage. Trends Plant Sci. doi:10.1016/j.tplants.2015.05.010
2014
Coursol S, Fromentin J, Noirot E, Brière C, Robert F, Morel J, Liang Y-K, Lherminier J, Simon-Plas F (2014) Long-chain bases and their phosphorylated derivatives differentially regulate cryptogein-induced production of reactive oxygen species in tobacco BY-2 cells. New Phytologist 205(3), 1239-49.
Kittang A, Iversen T, Fossum K, Mazars C, CarneroDiaz E, Boucheron−Dubuisson E, Le Disquet I, Herranz R, Pereda−Loth V, Medina FJ (2014) Exploration of plant growth and development using the European Modular Cultivation System facility on the International Space Station. Plant Biology 16(3), 528-538.
Mazars C, Brière C, Grat S, Pichereaux C, Rossignol M, Pereda-Loth V, Eche B, Boucheron-Dubuisson E, Le Disquet I, Medina FJ, Graziana A, Carnero-Diaz E (2014) Microgravity induces changes in microsome-associated proteins of Arabidopsis seedlings grown on board the international space station. PLoS One 9(3) : e91814. doi:10.1371/journal.pone.0091814
Mazars C, Brière C, Grat S, Pichereaux C, Rossignol M, Pereda-Loth V, Eche B, Boucheron-Dubuisson E, Le Disquet I, Medina FJ, Graziana A, Carnero-Diaz E (2014) Microsome-associated proteome modifications of Arabidopsis seedlings grown on board the International Space Station reveal the possible effect on plants of space stresses other than microgravity. Plant Signal. Behav. 9. e29637
Ronzier E, Sanchez C-F, Prado K, Briere C, Leonhard N, Thibaud J-B, Xiong T-C (2014). CPK13, a non-canonical CPK, specifically inhibits KAT2 and KAT1 Shaker channels and reduces stomatal opening. Plant Physiol. doi:10.1104/pp.114.240226
Xiong TC, Ronzier E, Sanchez F, Corratgé-Faillie C, Mazars C, Thibaud JB (2014) Imaging long distance propagating calcium signals in intact plant leaves with the BRET-based GFP-aequorin reporter. Front Plant Sci 5:43. doi:10.3389/fpls.2014.00043
2013
Cheval C, Aldon D, Galaud JP, Ranty B (2013) Calcium/calmodulin-mediated regulation of plant immunity. Biochimica et Biophysica Acta 1833, 1766-1771.
Lachaud C, Prigent E, Thuleau P, Grat S, Da Silva D, Brière C, Mazars C, Cotelle V (2013) 14-3-3-regulated Ca2+-dependent protein kinase CPK3 is required for sphingolipid-induced cell death in Arabidopsis Cell Death and Differentiation 20(2), 209-217.
Pujol B, Galaud JP (2013) A practical guide to quantifying the effect of genes underlying adaptation in a mixed genomics and evolutionary ecology approach. Acta Botanica Gallica 160, 197-204.
Thuleau P, Aldon D, Cotelle V, Briere C, Ranty B, Galaud JP, Mazars C (2013) Relationships between calcium and sphingolipid-dependent signalling pathways during the early steps of plant-pathogen interactions. Biochimica et Biophysica Acta 1833, 1590-1594.
2012
Leba LJ, Perochon A, Cheval C, Ranty B, Galaud JP, Aldon D (2012) CML9, a multifunctional Arabidopsis thaliana calmodulin-like protein involved in stress responses and plant growth ? Plant Signal Behav. 7(9). PMID : 22899061.
Leba LJ, Cheval C, Ortiz-Martín I, Ranty B, Beuzón CR, Galaud JP, Aldon D (2012) CML9, an Arabidopsis calmodulin-like protein, contributes to plant innate immunity through a flagellin-dependent signalling pathway. Plant J. 71(6):976-89. PubMed PMID : 22563930.
Ranty B, Cotelle V, Galaud JP., Mazars C (2012) Nuclear calcium signaling and its involvement in transcriptional regulation in plants. Adv Exp Med Biol 740:1123-43. Invited review
Thuleau P, Briere C, Mazars C (2012) Recent advances in plant cell nuclear signalling. Mol Plant. 5(5):968-70. Invited Highlight.
2011
Amelot N, Dorlhac de Borne F, San Clemente H, Grima-Pettenati J, Mazars C, Brière C (2011) Transcriptome analysis of tobacco BY-2 cells elicited by cryptogein reveals new potential actors of calcium-dependent and calcium-independent plant defense pathways. Cell Calcium. 51(2):117-30
Amelot N, Carrouche A, Danoun S, Bourque S, Haiech J, Pugin A, Ranjeva R, Grima-Pettenati J, Mazars C, Briere C (2011) Cryptogein, a fungal elicitor, remodels the phenylpropanoid metabolism of tobacco cell suspension cultures in a calcium-dependent manner. Plant Cell Env 34 : 149-161 
Aubert Y, Leba LJ, Cheval C, Ranty B, Vavasseur A, Aldon D, Galaud JP (2011) Involvement of RD20, a member of caleaosin family, in ABA-mediated regulation of germination in Arabidopsis thaliana. Plant Signal & Behav. 6 : 538-540
Canonne J, Marino D, Jauneau A, Pouzet C, Brière C, Roby D, Rivas S (2011) The Xanthomonas Type III Effector XopD Targets the Arabidopsis Transcription Factor MYB30 to Suppress Plant Defense. Plant Cell 23 : 3498-3511
Da Silva D, Lachaud C, Cotelle V, Briere C, Grat S, Mazars C, Thuleau P (2011) Nitric oxide production is not required for dihydrosphingosine-induced cell death in tobacco BY-2 cells. Plant Signal & Behav. 6(5) : 736-739
Lachaud C, Da Silva D, Amelot N, Beziat C, Briere C, Cotelle V, Graziana A, Grat S, Mazars C, Thuleau P (2011) Dihydrosphingosine-induced programmed cell death in tobacco BY-2 Cells is independent of H2O2 production. Mol Plant 4(2) : 310-318
Masclaux F, Galaud JP (2011) Heat-inducible RNAi for gene functional analysis in plants. In Methods in Molecular Biology “RNAi and Plant Gene Function Analysis. Methods and Protocols”, Vol. 744. Humana Press, pp37-55
Mazars C, Brière C, Bourque S, Thuleau P (2011) Nuclear calcium signaling : an emerging topic in plants. Biochimie 93(12) : 2068-2074
Mazars C, Thuleau P, Cotelle V, Brière C (2011) Calcium signaling and homeostasis in nuclei. In S Luan, ed, Coding and decoding of calcium signals in plants, Springer, pp7-24
Perochon A, Aldon D, Galaud JP, Ranty B (2011) Calmodulin and calmodulin-like proteins in plant calcium signaling. Biochimie 93(12) : 2048-2053
Ranty B, Cotelle V, Galaud JP, Mazars C (2011) Nuclear calcium signaling and its involvement in transcriptional regulation in plants. In S Islam, ed, Calcium signaling Series : Advances In Experimental Medecine and Biology, vol. 740 ch. 50, Springer
2006
Briere C, Xiong TC, Mazars C, Ranjeva R (2006) Autonomous regulation of free Ca2+ concentrations in isolated plant cell nuclei : a mathematical analysis. Cell Calcium 39 : 293-303
Garnier L, Simon-Plas F, Thuleau P, Agnel JP, Blein JP, Ranjeva R, Montillet JL (2006) Cadmium affects tobacco cells by a series of three waves of reactive oxygen species that contribute to cytotoxicity. Plant Cell Environ 29 : 1956-1969
Gaulin E, Drame N, Lafitte C, Torto-Alalibo T, Martinez Y, Ameline-Torregrosa C, Khatib M, Mazarguil H, Villalba-Mateos F, Kamoun S, Mazars C, Dumas B, Bottin A, Esquerre-Tugaye MT, Rickauer M (2006) Cellulose binding domains of a Phytophthora cell wall protein are novel pathogen-associated molecular patterns. Plant Cell 18 : 1766-1777
Hogg BV, Cullimore JV, Ranjeva R, Bono JJ (2006) The DMI1 and DMI2 early symbiotic genes of medicago truncatula are required for a high-affinity nodulation factor-binding site associated to a particulate fraction of roots. Plant Physiol 140 : 365-373
Lannoo N, Peumans WJ, Pamel EV, Alvarez R, Xiong TC, Hause G, Mazars C, Van Damme EJ (2006) Localization and in vitro binding studies suggest that the cytoplasmic/nuclear tobacco lectin can interact in situ with high-mannose and complex N-glycans. FEBS Lett 580 : 6329-6337
Laugesen S, Messinese E, Hem S, Pichereaux C, Grat S, Ranjeva R, Rossignol M, Bono JJ (2006) Phosphoproteins analysis in plants : a proteomic approach. Phytochem 67 : 2208-2214
Lecourieux D, Ranjeva R, Pugin A (2006) Calcium in plant defence-signalling pathways. New Phytol 171 : 249-269
Moreau M, Ranjeva R (2006) The calcium, an object of study, astonishing, no ? Med Sci (Paris) 22 : 1019
Ranty B, Aldon D, Galaud JP (2006) Plant Calmodulins and Calmodulin-Related Proteins : Multifaceted Relays to Decode Calcium Signals. Plant Signaling & Behavior 1 : 96-104
Xiong TC, Bourque S, Lecourieux D, Amelot N, Grat S, Briere C, Mazars C, Pugin A, Ranjeva R (2006) Calcium signaling in plant cell organelles delimited by a double membrane. Biochim Biophys Acta 1763 : 1209-1215
Xiong TC, Bourque S, Mazars C, Pugin A, Ranjeva R (2006) Cytosolic and nuclear calcium signalling in plants reply to biotic and abiotic stimuli. Med Sci (Paris) 22 : 1025-1028
2005
Leclercq J, Ranty B, Sanchez-Ballesta MT, Li Z, Jones B, Jauneau A, Pech JC, Latche A, Ranjeva R, Bouzayen M (2005) Molecular and biochemical characterization of LeCRK1, a ripening-associated tomato CDPK-related kinase. J Exp Bot 56 : 25-35
Lecourieux D, Lamotte O, Bourque S, Wendehenne D, Mazars C, Ranjeva R, Pugin A (2005) Proteinaceous and oligosaccharidic elicitors induce different calcium signatures in the nucleus of tobacco cells. Cell Calcium 38 : 527-538
Masclaux FG, Galaud JP, Pont-Lezica R (2005) The riddle of the plant vacuolar sorting receptors. Protoplasma 226 : 103-108
Masclaux FG, Pont-Lezica R, Galaud JP (2005) Relationship between allelic state of T-DNA and DNA methylation of chromosomal integration region in transformed Arabidopsis thaliana plants. Plant Mol Biol 58 : 295-303
Olah B, Briere C, Becard G, Denarie J, Gough C (2005) Nod factors and a diffusible factor from arbuscular mycorrhizal fungi stimulate lateral root formation in Medicago truncatula via the DMI1/DMI2 signalling pathway. Plant J 44 : 195-207
2004
Charpenteau M, Jaworski K, Ramirez BC, Tretyn A, Ranjeva R, Ranty B (2004) A receptor-like kinase from Arabidopsis thaliana is a calmodulin-binding protein. Biochem J 379 : 841-848
Gressent F, Cullimore JV, Ranjeva R, Bono JJ (2004) Radiolabeling of lipo-chitooligosaccharides using the NodH sulfotransferase : a two-step enzymatic procedure. BMC Biochem 5 : 4
Masclaux F, Charpenteau M, Takahashi T, Pont-Lezica R, Galaud JP (2004) Gene silencing using a heat-inducible RNAi system in Arabidopsis. Biochem Biophys Res Commun 321 : 364-369
Perruc E, Charpenteau M, Ramirez BC, Jauneau A, Galaud JP, Ranjeva R, Ranty B (2004) A novel calmodulin-binding protein functions as a negative regulator of osmotic stress tolerance in Arabidopsis thaliana seedlings. Plant J 38 : 410-420
Xiong TC, Jauneau A, Ranjeva R, Mazars C (2004) Isolated plant nuclei as mechanical and thermal sensors involved in calcium signalling. Plant J 40 : 12-21
2003
Centis-Aubay S, Gasset G, Mazars C, Ranjeva R, Graziana A (2003) Changes in gravitational forces induce modifications of gene expression in A. thaliana seedlings. Planta 218 : 179-185
Laval V, Masclaux F, Serin A, Carriere M, Roldan C, Devic M, Pont-Lezica RF, Galaud JP (2003) Seed germination is blocked in Arabidopsis putative vacuolar sorting receptor (atbp80) antisense transformants. J Exp Bot 54 : 213-221
Thuleau P, Leclerc C, Xiong TC, Mazars C, Leclerc C, Moreau M (2003) Luminous plant and animals or the expression of aequorin and “chameleon” probes : a new light in calcium signaling. J Soc Biol 197 : 291-300
2010
Aubert Y, Vile D, Pervent M, Aldon D, Ranty B, Simonneau T, Vavasseur A, Galaud JP (2010) RD20, a Stress-inducible caleosin, participates in stomatal control, transpiration and drought tolerance in Arabidopsis thaliana. Plant Cell Physiol 51 : 1975-1987
Borges JP, Culerrier R, Aldon D, Barre A, Benoist H, Saurel O, Milon A, Didier A, Rouge P (2010) GATEWAY technology and E. coli recombinant system produce a properly folded and functional recombinant allergen of the lipid transfer protein of apple (Mal d 3). Protein Expr Purif 70 : 277-282
Dahan J, Wendehenne D, Ranjeva R, Pugin A, Bourque S (2010) Nuclear protein kinases : still enigmatic components in plant cell signalling. New Phytol 185 : 355-368
Froidure S, Canonne J, Daniel X, Jauneau A, Briere C, Roby D, Rivas S (2010) AtsPLA2-alpha nuclear relocalization by the Arabidopsis transcription factor AtMYB30 leads to repression of the plant defense response. Proc Natl Acad Sci U S A 107 : 15281-15286
Lachaud C, Da Silva D, Cotelle V, Thuleau P, Xiong TC, Jauneau A, Briere C, Graziana A, Bellec Y, Faure JD, Ranjeva R, Mazars C (2010) Nuclear calcium controls the apoptotic-like cell death induced by d-erythro-sphinganine in tobacco cells. Cell Calcium 47 : 92-100
Mazars C, Thuleau P, Lamotte O, Bourque S (2010) Cross-talk between ROS and calcium in regulation of nuclear activities. Mol Plant 3 : 706-718
Perochon A, Dieterle S, Pouzet C, Aldon D, Galaud JP, Ranty B (2010) Interaction of a plant pseudo-response regulator with a calmodulin-like protein. Biochem Biophys Res Commun. 398(4) : 747-751
Tasset C, Bernoux M, Jauneau A, Pouzet C, Brière C, Kieffer-Jacquinod S, Rivas S, Marco Y, Deslandes L (2010) Autoacetylation of the Ralstonia solanacearum effector PopP2 targets a lysine residue essential for RRS1-R-mediated immunity in Arabidopsis. PLoS Pathog 6 : e1001202
2009
Dagher R, Briere C, Feve M, Zeniou M, Pigault C, Mazars C, Chneiweiss H, Ranjeva R, Kilhoffer MC, Haiech J (2009) Calcium fingerprints induced by calmodulin interactors in eukaryotic cells. Biochim Biophys Acta 1793 : 1068-1077
Mazars C, Bourque S, Mithofer A, Pugin A, Ranjeva R (2009) Calcium homeostasis in plant cell nuclei. New Phytol 181 : 261-274
Mithofer A, Mazars C, Maffei ME (2009) Probing Spatio-temporal Intracellular Calcium Variations in Plants. Meth Mol Biol 479 : 79-92
Poutrain P, Mazars C, Thiersault M, Rideau M, Pichon O (2009) Two distinct intracellular Ca2+-release components act in opposite ways in the regulation of the auxin-dependent MIA biosynthesis in Catharanthus roseus cells. J Exp Bot 60 : 1387-1398
Vadassery J, Ranf S, Drzewiecki C, Mithofer A, Mazars C, Scheel D, Lee J, Oelmuller R (2009) A cell wall extract from the endophytic fungus Piriformospora indica promotes growth of Arabidopsis seedlings and induces intracellular calcium elevation in roots. Plant J 59 : 193-206
2008
Bernoux M, Timmers T, Jauneau A, Briere C, de Wit PJ, Marco Y, Deslandes L (2008) RD19, an Arabidopsis cysteine protease required for RRS1-R-mediated resistance, is relocalized to the nucleus by the Ralstonia solanacearum PopP2 effector. Plant Cell 20 : 2252-2264
Magnan F, Ranty B, Charpenteau M, Sotta B, Galaud JP, Aldon D (2008) Mutations in AtCML9, a calmodulin-like protein from Arabidopsis thaliana, alter plant responses to abiotic stress and abscisic acid. Plant J 56 : 575-589
Xiong TC, Coursol S, Grat S, Ranjeva R, Mazars C (2008) Sphingolipid metabolites selectively elicit increases in nuclear calcium concentration in cell suspension cultures and in isolated nuclei of tobacco. Cell Calcium 43 : 29-37
2007
Parre E, Ghars MA, Leprince AS, Thiery L, Lefebvre D, Bordenave M, Richard L, Mazars C, Abdelly C, Savoure A (2007) Calcium signaling via phospholipase C is essential for proline accumulation upon ionic but not nonionic hyperosmotic stresses in Arabidopsis. Plant Physiol 144 : 503-512
Ranty B, Aldon D, Galaud JP (2007) Regulation of gene expression by calmodulin in plants. Med Sci (Paris) 23 : 13-14
Walter A, Mazars C, Maitrejean M, Hopke J, Ranjeva R, Boland W, Mithofer A (2007) Structural requirements of jasmonates and synthetic analogues as inducers of Ca2+ signals in the nucleus and the cytosol of plant cells. Angew Chem Int Ed Engl 46 : 4783-4785
2021
Plant J. 2021 Ethylene signaling modulates tomato pollen tube growth through modifications of cell wall remodeling and calcium gradient. Aug;107(3):893-908. doi: 10.1111/tpj.15353. Epub 2021 Jun 20.
The Same against Many: AtCML8, a Ca2+ Sensor Acting as a Positive Regulator of Defense Responses against Several Plant Pathogens. Int J Mol Sci. 2021 Sep 28;22(19):10469. doi: 10.3390/ijms221910469.
Xiong T, Tan Q, Li S, Mazars C, Galaud JP, Zhu X., 2021. Interactions between calcium and ABA signaling pathways in the regulation of fruit ripening. J Plant Physiol. 2021 doi: 10.1016/j.jplph.2020.153309.
Ca2+-Dependent Protein Kinase 6 Enhances KAT2 Shaker Channel Activity in Arabidopsis thaliana. Int J Mol Sci. 2021 Feb 5;22(4):1596. doi: 10.3390/ijms22041596.
Kusch S, Larrouy J, Ibrahim HMM, Mounichetty S, Gasset N, Navaud O, Mbengue M, Zanchetta C, Lopez-Roques C, Donnadieu C, Godiard L, Raffaele S., 2021. Transcriptional response to host chemical cues underpins the expansion of host range in a fungal plant pathogen lineage. The ISME Journal 16, 138–148
2022
Carpentier S, Aldon D, Berthomé R, Galaud JP, 2022. Is there a specific calcium signal out there to decode combined biotic stress and temperature elevation? Front Plant Sci. doi: 10.3389/fpls.2022.1004406..
2023
Ferrer-Orgaz S, Tiwari M, Isidra-Arellano MC, Pozas-Rodriguez EA, Vernié T, Rich MK, Mbengue M, Formey D, Delaux PM, Ané JM, Valdés-López O., 2023. Early Phosphorylated Protein 1 is required to activate the early rhizobial infection program. New Phytologist 241, 962–968.
Liu C, Mentzelopoulou A, Papagavriil F, Ramachandran P, Perraki A, Claus L, Barg S, Dörmann P, Jaillais Y, Johnen P, Russinova E, Gizeli E, Schaaf G, Moschou PN., 2023. SEC14-like condensate phase transitions at plasma membranes regulate root growth in Arabidopsis. PLoS Biol. 18;21(9):e3002305. doi: 10.1371/journal.pbio.3002305.
Goto, Yukihisa, Yasuhiro Kadota, Malick Mbengue, Jennifer D Lewis, Hidenori Matsui, Noriko Maki, Jan Sklenar, et al. « The leucine-rich repeat receptor kinase QSK1 is a novel regulator of PRR-RBOHD complex and is employed by the bacterial effector HopF2Ptoto modulate plant immunity », (2023). https://doi.org/10.1101/2023.12.13.571443. Preprint.
2024
Zhu Q., Tan Q., Gao Q., Zheng S., Chen W., Galaud J.P., Li X., Zhu X., 2024. Calmodulin-like protein CML15 interacts with PP2C46/65 to regulate papaya fruit ripening via integrating calcium, ABA and ethylene signals. In press in Plant Biotechnology Journal. DOI: 10.1111/pbi.14297.
Teyssier, E., Grat, S., Rich, M., Delaux, P.-M. & Mbengue, M. LysM-RLK plays an ancestral symbiotic function in plants. (2024) doi:10.1101/2024.01.16.575821. Preprint.
Vernié, T., Rich M., Pellen T., Teyssier E., Garrigues V., Chauderon L., Medioni L., van Beveren F., Libourel C., Keller J., Girou C., Lefort C., Le Ru A., Reinhardt D., Kodama K., Shimazaki S., Morel P., Kyozuka J., Mbengue M., Vandenbussche M., Delaux P.M. Conservation of symbiotic signalling across 450 million years of plant evolution. (2024) doi:10.1101/2024.01.16.575147. Preprint.
2020
Barbacci A, Navaud O, Mbengue M, Barascud M, Godiard L, Khafif M, Lacaze A, Raffaele S. (2020) Rapid identification of an Arabidopsis NLR gene as a candidate conferring susceptibility to Sclerotinia sclerotiorum using time-resolved automated phenotyping. Plant J. 2020 Jul;103(2):903-917. doi: 10.1111/tpj.14747.
Sucher J, Mbengue M, Dresen A, Barascud M, Didelon M, Barbacci A, Raffaele S. (2020) Phylotranscriptomics of the Pentapetalae Reveals Frequent Regulatory Variation in Plant Local Responses to the Fungal Pathogen Sclerotinia sclerotiorum. Plant Cell. 2020 Jun;32(6):1820-1844. doi: 10.1105/tpc.19.00806.
Derbyshire M, Mbengue M, Barascud M, Navaud O, Raffaele S. (2020) Small RNAs from the plant pathogenic fungus Sclerotinia sclerotiorum highlight host candidate genes associated with quantitative disease resistance. Mol Plant Pathol. 2019 Sep;20(9):1279-1297. doi: 10.1111/mpp.12841.
2019
Dell’Aglio E, Giustini C, Kraut A, Couté Y, Costa A, Decros G, Gibon Y, Mazars C, Matringe M, Finazzi G, Curien G. (2019) Identification of the Arabidopsis Calmodulin-Dependent NAD+ Kinase That Sustains the Elicitor-Induced Oxidative Burst. Plant Physiol. 181(4):1449-1458.
Bourque S, Lindermayr C, Mazars C., (2019) Post-translational Modifications in Plant Nuclear Signaling: Novel Insights Into Responses to Environmental Changes. Front Plant Sci. 2019 Feb 11;10:104. doi: 10.3389/fpls.2019.00104
Peyraud R, Mbengue M, Barbacci A, Raffaele S (2019) Intercellular cooperation in a fungal plant pathogenfacilitates host colonization. Proc Natl Acad Sci USA 116 (8) 3193-3201
2018
Aldon D, Mbengue M, Mazars C, Galaud JP (2018) Calcium Signalling in Plant Biotic Interactions. Int J Mol Sci. [http://www.mdpi.com/1422-0067/19/3/665]
Favre L., Ortalo-Magné A., Pichereaux C., Gargaros A., Burlet-Schiltz O., Cotelle V. and Culioli G. (2018). Metabolome and proteome changes between biofilm and planktonic phenotypes of the marine bacterium Pseudoalteromonas lipolytica TC8. Biofouling. 34 : 132-148.
Heyer M, Scholz SS, Voigt D, Reichelt M, Aldon D, Oelmüller R, Boland W, Mithöfer A., 2018. Herbivory-responsive calmodulin-like protein CML9 does not guide jasmonate-mediated defenses in Arabidopsis thaliana. PLoS One. 16;13(5):e0197633. [https://pubmed.ncbi.nlm.nih.gov/29768484/]
2017
Zhu X, Perez M, Didier Aldon D & JP Galaud (2017) Respective contribution of CML8 and CML9, two arabidopsis calmodulin-like proteins, to plant stress responses.[http://www.tandfonline.com/doi/full…]
Ghorbel M., Cotelle V., Ebel C., Zaidi I., Ormancey M., Galaud J.P., Hanin M. (2017) Regulation of the wheat MAP kinase phosphatase 1 by 14-3-3 proteins. [http://www.sciencedirect.com/scienc…] 257, 37–47.
Zhu X, Robe E, Jomat L, Aldon A, Mazars C, Galaud JP (2017) Calmodulin-like 8 contributes to plant immunity.[https://academic.oup.com/pcp/articl…] 58(2):307-319
Ormancey M, Thuleau P, Mazars C, Cotelle V (2017) CDPKs and 14-3-3 Proteins : Emerging Duo in Signaling. Trends Plant Sci. S1360-1385(16)30190-X.
Badet T*, Peyraud R*, Mbengue M*, Navaud O, Derbyshire M, Oliver RP, Barbacci A, Raffaele S (2017) Codon optimization underpins generalist parasitism in fungi. Elife
Badet T, Voisin D, Mbengue M, Barascud M, Sucher J, Sadon P, Balague C, Roby D, Raffaele S (2017) Parallel evolution of the POQR prolyl oligo peptidase gene conferring plant quantitative disease resistance. PLoS Genet 13: e1007143
Bucherl CA, Jarsch IK, Schudoma C, Segonzac C, Mbengue M, Robatzek S, MacLean D, Ott T, Zipfel C (2017) Plant immune and growth receptors share common signalling components but localise to distinct plasma membrane nanodomains. Elife 6
Derbyshire M, Denton-Giles M, Hegedus D, Seifbarghy S, Rollins J, van Kan J, Seidl MF, Faino L, Mbengue M, Navaud O, Raffaele S, Hammond-Kosack K, Heard S, Oliver R (2017) The complete genome sequence of the phytopathogenic fungus Sclerotinia sclerotiorum reveals insights into the genome architecture of broad host range pathogens. Genome Biol Evol 9: 593-618
2016
Testard A, Da Silva D, Ormancey M, Pichereaux C, Pouzet C, Jauneau A, Grat S, Robe E, Brière C, Cotelle V, Mazars C, Thuleau P (2016) Calcium- and Nitric Oxide-Dependent Nuclear Accumulation of Cytosolic Glyceraldehyde-3-Phosphate Dehydrogenase in Response to Long Chain Bases in Tobacco BY-2 Cells. Plant Cell Physiol 57 (10) :2221-2231
Ranty B, Aldon D, Cotelle V, Galaud JP, Thuleau P, Mazars C (2016) Calcium sensors as key hubs in plant responses to biotic and abiotic stresses Front. Plant Sci 7:327
Cotelle V, Leonhardt N (2016) 14-3-3 proteins in guard cell signaling. Front.Plant Sci 6:12
Mazars C ; Ranty B ; Aldon D ; Oelmüller R ; Galaud J-P ; Mithöfer A (2016) CMLs control host-plant interactions.Endocytobiosis and Cell Research 27:13-19
Mbengue M*, Bourdais G*, Gervasi F, Beck M, Zhou J, Spallek T, Bartels S, Boller T, Ueda T, Kuhn H, Robatzek S (2016) Clathrin-dependent endocytosis is required for immunity mediated by pattern recognition receptor kinases. Proc Natl Acad Sci U S A 113: 11034-11039
Mbengue M, Navaud O, Peyraud R, Barascud M, Badet T, Vincent R, Barbacci A, Raffaele S (2016) Emerging Trends in Molecular Interactions between Plants and the Broad Host Range Fungal Pathogens Botrytis cinerea and Sclerotinia sclerotiorum. Front Plant Sci 7: 422
Mithoe SC, Ludwig C, Pel MJ, Cucinotta M, Casartelli A, Mbengue M, Sklenar J, Derbyshire P, Robatzek S, Pieterse CM, Aebersold R, Menke FL (2016) Attenuation of pattern recognition receptor signaling is mediated by a MAP kinase kinase kinase. EMBO Rep 17: 441-454
Postma J, Liebrand TW, Bi G, Evrard A, Bye RR, Mbengue M, Kuhn H, Joosten MH, Robatzek S (2016) Avr4 promotes Cf-4 receptor-like protein association with the BAK1/SERK3 receptor-like kinase to initiate receptor endocytosis and plant immunity. New Phytol 210: 627-642
Vernie T, Camut S, Camps C, Rembliere C, de Carvalho-Niebel F, Mbengue M, Timmers T, Gasciolli V, Thompson R, le Signor C, Lefebvre B, Cullimore J, Herve C (2016) PUB1 Interacts with the Receptor Kinase DMI2 and Negatively Regulates Rhizobial and Arbuscular Mycorrhizal Symbioses through Its Ubiquitination Activity in Medicago truncatula. Plant Physiol 170: 2312-2324
2015
Ghorbel M, Zaidi I, Robe E, Ranty B, Mazars C, Galaud JP, Hanin M (2015) The activity of the wheat MAP kinase phosphatase 1 is regulated by manganese and by calmodulin. Biochimie 108 : 13-19
Zhu X, Dunand C, Snedden W, Galaud JP (2015) CaM and CML emergence in the green lineage. Trends Plant Sci. doi:10.1016/j.tplants.2015.05.010
2014
Coursol S, Fromentin J, Noirot E, Brière C, Robert F, Morel J, Liang Y-K, Lherminier J, Simon-Plas F (2014) Long-chain bases and their phosphorylated derivatives differentially regulate cryptogein-induced production of reactive oxygen species in tobacco BY-2 cells. New Phytologist 205(3), 1239-49.
Kittang A, Iversen T, Fossum K, Mazars C, CarneroDiaz E, Boucheron−Dubuisson E, Le Disquet I, Herranz R, Pereda−Loth V, Medina FJ (2014) Exploration of plant growth and development using the European Modular Cultivation System facility on the International Space Station. Plant Biology 16(3), 528-538.
Mazars C, Brière C, Grat S, Pichereaux C, Rossignol M, Pereda-Loth V, Eche B, Boucheron-Dubuisson E, Le Disquet I, Medina FJ, Graziana A, Carnero-Diaz E (2014) Microgravity induces changes in microsome-associated proteins of Arabidopsis seedlings grown on board the international space station. PLoS One 9(3) : e91814. doi:10.1371/journal.pone.0091814
Mazars C, Brière C, Grat S, Pichereaux C, Rossignol M, Pereda-Loth V, Eche B, Boucheron-Dubuisson E, Le Disquet I, Medina FJ, Graziana A, Carnero-Diaz E (2014) Microsome-associated proteome modifications of Arabidopsis seedlings grown on board the International Space Station reveal the possible effect on plants of space stresses other than microgravity. Plant Signal. Behav. 9. e29637
Ronzier E, Sanchez C-F, Prado K, Briere C, Leonhard N, Thibaud J-B, Xiong T-C (2014). CPK13, a non-canonical CPK, specifically inhibits KAT2 and KAT1 Shaker channels and reduces stomatal opening. Plant Physiol. doi:10.1104/pp.114.240226
Xiong TC, Ronzier E, Sanchez F, Corratgé-Faillie C, Mazars C, Thibaud JB (2014) Imaging long distance propagating calcium signals in intact plant leaves with the BRET-based GFP-aequorin reporter. Front Plant Sci 5:43. doi:10.3389/fpls.2014.00043
2013
Cheval C, Aldon D, Galaud JP, Ranty B (2013) Calcium/calmodulin-mediated regulation of plant immunity. Biochimica et Biophysica Acta 1833, 1766-1771.
Lachaud C, Prigent E, Thuleau P, Grat S, Da Silva D, Brière C, Mazars C, Cotelle V (2013) 14-3-3-regulated Ca2+-dependent protein kinase CPK3 is required for sphingolipid-induced cell death in Arabidopsis Cell Death and Differentiation 20(2), 209-217.
Pujol B, Galaud JP (2013) A practical guide to quantifying the effect of genes underlying adaptation in a mixed genomics and evolutionary ecology approach. Acta Botanica Gallica 160, 197-204.
Thuleau P, Aldon D, Cotelle V, Briere C, Ranty B, Galaud JP, Mazars C (2013) Relationships between calcium and sphingolipid-dependent signalling pathways during the early steps of plant-pathogen interactions. Biochimica et Biophysica Acta 1833, 1590-1594.
2012
Leba LJ, Perochon A, Cheval C, Ranty B, Galaud JP, Aldon D (2012) CML9, a multifunctional Arabidopsis thaliana calmodulin-like protein involved in stress responses and plant growth ? Plant Signal Behav. 7(9). PMID : 22899061.
Leba LJ, Cheval C, Ortiz-Martín I, Ranty B, Beuzón CR, Galaud JP, Aldon D (2012) CML9, an Arabidopsis calmodulin-like protein, contributes to plant innate immunity through a flagellin-dependent signalling pathway. Plant J. 71(6):976-89. PubMed PMID : 22563930.
Ranty B, Cotelle V, Galaud JP., Mazars C (2012) Nuclear calcium signaling and its involvement in transcriptional regulation in plants. Adv Exp Med Biol 740:1123-43. Invited review
Thuleau P, Briere C, Mazars C (2012) Recent advances in plant cell nuclear signalling. Mol Plant. 5(5):968-70. Invited Highlight.
2011
Amelot N, Dorlhac de Borne F, San Clemente H, Grima-Pettenati J, Mazars C, Brière C (2011) Transcriptome analysis of tobacco BY-2 cells elicited by cryptogein reveals new potential actors of calcium-dependent and calcium-independent plant defense pathways. Cell Calcium. 51(2):117-30
Amelot N, Carrouche A, Danoun S, Bourque S, Haiech J, Pugin A, Ranjeva R, Grima-Pettenati J, Mazars C, Briere C (2011) Cryptogein, a fungal elicitor, remodels the phenylpropanoid metabolism of tobacco cell suspension cultures in a calcium-dependent manner. Plant Cell Env 34 : 149-161
Aubert Y, Leba LJ, Cheval C, Ranty B, Vavasseur A, Aldon D, Galaud JP (2011) Involvement of RD20, a member of caleaosin family, in ABA-mediated regulation of germination in Arabidopsis thaliana. Plant Signal & Behav. 6 : 538-540
Canonne J, Marino D, Jauneau A, Pouzet C, Brière C, Roby D, Rivas S (2011) The Xanthomonas Type III Effector XopD Targets the Arabidopsis Transcription Factor MYB30 to Suppress Plant Defense. Plant Cell 23 : 3498-3511
Da Silva D, Lachaud C, Cotelle V, Briere C, Grat S, Mazars C, Thuleau P (2011) Nitric oxide production is not required for dihydrosphingosine-induced cell death in tobacco BY-2 cells. Plant Signal & Behav. 6(5) : 736-739
Lachaud C, Da Silva D, Amelot N, Beziat C, Briere C, Cotelle V, Graziana A, Grat S, Mazars C, Thuleau P (2011) Dihydrosphingosine-induced programmed cell death in tobacco BY-2 Cells is independent of H2O2 production. Mol Plant 4(2) : 310-318
Masclaux F, Galaud JP (2011) Heat-inducible RNAi for gene functional analysis in plants. In Methods in Molecular Biology “RNAi and Plant Gene Function Analysis. Methods and Protocols”, Vol. 744. Humana Press, pp37-55
Mazars C, Brière C, Bourque S, Thuleau P (2011) Nuclear calcium signaling : an emerging topic in plants. Biochimie 93(12) : 2068-2074
Mazars C, Thuleau P, Cotelle V, Brière C (2011) Calcium signaling and homeostasis in nuclei. In S Luan, ed, Coding and decoding of calcium signals in plants, Springer, pp7-24
Perochon A, Aldon D, Galaud JP, Ranty B (2011) Calmodulin and calmodulin-like proteins in plant calcium signaling. Biochimie 93(12) : 2048-2053
Ranty B, Cotelle V, Galaud JP, Mazars C (2011) Nuclear calcium signaling and its involvement in transcriptional regulation in plants. In S Islam, ed, Calcium signaling Series : Advances In Experimental Medecine and Biology, vol. 740 ch. 50, Springer
2006
Briere C, Xiong TC, Mazars C, Ranjeva R (2006) Autonomous regulation of free Ca2+ concentrations in isolated plant cell nuclei : a mathematical analysis. Cell Calcium 39 : 293-303
Garnier L, Simon-Plas F, Thuleau P, Agnel JP, Blein JP, Ranjeva R, Montillet JL (2006) Cadmium affects tobacco cells by a series of three waves of reactive oxygen species that contribute to cytotoxicity. Plant Cell Environ 29 : 1956-1969
Gaulin E, Drame N, Lafitte C, Torto-Alalibo T, Martinez Y, Ameline-Torregrosa C, Khatib M, Mazarguil H, Villalba-Mateos F, Kamoun S, Mazars C, Dumas B, Bottin A, Esquerre-Tugaye MT, Rickauer M (2006) Cellulose binding domains of a Phytophthora cell wall protein are novel pathogen-associated molecular patterns. Plant Cell 18 : 1766-1777
Hogg BV, Cullimore JV, Ranjeva R, Bono JJ (2006) The DMI1 and DMI2 early symbiotic genes of medicago truncatula are required for a high-affinity nodulation factor-binding site associated to a particulate fraction of roots. Plant Physiol 140 : 365-373
Lannoo N, Peumans WJ, Pamel EV, Alvarez R, Xiong TC, Hause G, Mazars C, Van Damme EJ (2006) Localization and in vitro binding studies suggest that the cytoplasmic/nuclear tobacco lectin can interact in situ with high-mannose and complex N-glycans. FEBS Lett 580 : 6329-6337
Laugesen S, Messinese E, Hem S, Pichereaux C, Grat S, Ranjeva R, Rossignol M, Bono JJ (2006) Phosphoproteins analysis in plants : a proteomic approach. Phytochem 67 : 2208-2214
Lecourieux D, Ranjeva R, Pugin A (2006) Calcium in plant defence-signalling pathways. New Phytol 171 : 249-269
Moreau M, Ranjeva R (2006) The calcium, an object of study, astonishing, no ? Med Sci (Paris) 22 : 1019
Ranty B, Aldon D, Galaud JP (2006) Plant Calmodulins and Calmodulin-Related Proteins : Multifaceted Relays to Decode Calcium Signals. Plant Signaling & Behavior 1 : 96-104
Xiong TC, Bourque S, Lecourieux D, Amelot N, Grat S, Briere C, Mazars C, Pugin A, Ranjeva R (2006) Calcium signaling in plant cell organelles delimited by a double membrane. Biochim Biophys Acta 1763 : 1209-1215
Xiong TC, Bourque S, Mazars C, Pugin A, Ranjeva R (2006) Cytosolic and nuclear calcium signalling in plants reply to biotic and abiotic stimuli. Med Sci (Paris) 22 : 1025-1028
2005
Leclercq J, Ranty B, Sanchez-Ballesta MT, Li Z, Jones B, Jauneau A, Pech JC, Latche A, Ranjeva R, Bouzayen M (2005) Molecular and biochemical characterization of LeCRK1, a ripening-associated tomato CDPK-related kinase. J Exp Bot 56 : 25-35
Lecourieux D, Lamotte O, Bourque S, Wendehenne D, Mazars C, Ranjeva R, Pugin A (2005) Proteinaceous and oligosaccharidic elicitors induce different calcium signatures in the nucleus of tobacco cells. Cell Calcium 38 : 527-538
Masclaux FG, Galaud JP, Pont-Lezica R (2005) The riddle of the plant vacuolar sorting receptors. Protoplasma 226 : 103-108
Masclaux FG, Pont-Lezica R, Galaud JP (2005) Relationship between allelic state of T-DNA and DNA methylation of chromosomal integration region in transformed Arabidopsis thaliana plants. Plant Mol Biol 58 : 295-303
Olah B, Briere C, Becard G, Denarie J, Gough C (2005) Nod factors and a diffusible factor from arbuscular mycorrhizal fungi stimulate lateral root formation in Medicago truncatula via the DMI1/DMI2 signalling pathway. Plant J 44 : 195-207
2004
Charpenteau M, Jaworski K, Ramirez BC, Tretyn A, Ranjeva R, Ranty B (2004) A receptor-like kinase from Arabidopsis thaliana is a calmodulin-binding protein. Biochem J 379 : 841-848
Gressent F, Cullimore JV, Ranjeva R, Bono JJ (2004) Radiolabeling of lipo-chitooligosaccharides using the NodH sulfotransferase : a two-step enzymatic procedure. BMC Biochem 5 : 4
Masclaux F, Charpenteau M, Takahashi T, Pont-Lezica R, Galaud JP (2004) Gene silencing using a heat-inducible RNAi system in Arabidopsis. Biochem Biophys Res Commun 321 : 364-369
Perruc E, Charpenteau M, Ramirez BC, Jauneau A, Galaud JP, Ranjeva R, Ranty B (2004) A novel calmodulin-binding protein functions as a negative regulator of osmotic stress tolerance in Arabidopsis thaliana seedlings. Plant J 38 : 410-420
Xiong TC, Jauneau A, Ranjeva R, Mazars C (2004) Isolated plant nuclei as mechanical and thermal sensors involved in calcium signalling. Plant J 40 : 12-21
2003
Centis-Aubay S, Gasset G, Mazars C, Ranjeva R, Graziana A (2003) Changes in gravitational forces induce modifications of gene expression in A. thaliana seedlings. Planta 218 : 179-185
Laval V, Masclaux F, Serin A, Carriere M, Roldan C, Devic M, Pont-Lezica RF, Galaud JP (2003) Seed germination is blocked in Arabidopsis putative vacuolar sorting receptor (atbp80) antisense transformants. J Exp Bot 54 : 213-221
Thuleau P, Leclerc C, Xiong TC, Mazars C, Leclerc C, Moreau M (2003) Luminous plant and animals or the expression of aequorin and “chameleon” probes : a new light in calcium signaling. J Soc Biol 197 : 291-300
2010
Aubert Y, Vile D, Pervent M, Aldon D, Ranty B, Simonneau T, Vavasseur A, Galaud JP (2010) RD20, a Stress-inducible caleosin, participates in stomatal control, transpiration and drought tolerance in Arabidopsis thaliana. Plant Cell Physiol 51 : 1975-1987
Borges JP, Culerrier R, Aldon D, Barre A, Benoist H, Saurel O, Milon A, Didier A, Rouge P (2010) GATEWAY technology and E. coli recombinant system produce a properly folded and functional recombinant allergen of the lipid transfer protein of apple (Mal d 3). Protein Expr Purif 70 : 277-282
Dahan J, Wendehenne D, Ranjeva R, Pugin A, Bourque S (2010) Nuclear protein kinases : still enigmatic components in plant cell signalling. New Phytol 185 : 355-368
Froidure S, Canonne J, Daniel X, Jauneau A, Briere C, Roby D, Rivas S (2010) AtsPLA2-alpha nuclear relocalization by the Arabidopsis transcription factor AtMYB30 leads to repression of the plant defense response. Proc Natl Acad Sci U S A 107 : 15281-15286
Lachaud C, Da Silva D, Cotelle V, Thuleau P, Xiong TC, Jauneau A, Briere C, Graziana A, Bellec Y, Faure JD, Ranjeva R, Mazars C (2010) Nuclear calcium controls the apoptotic-like cell death induced by d-erythro-sphinganine in tobacco cells. Cell Calcium 47 : 92-100
Mazars C, Thuleau P, Lamotte O, Bourque S (2010) Cross-talk between ROS and calcium in regulation of nuclear activities. Mol Plant 3 : 706-718
Perochon A, Dieterle S, Pouzet C, Aldon D, Galaud JP, Ranty B (2010) Interaction of a plant pseudo-response regulator with a calmodulin-like protein. Biochem Biophys Res Commun. 398(4) : 747-751
Tasset C, Bernoux M, Jauneau A, Pouzet C, Brière C, Kieffer-Jacquinod S, Rivas S, Marco Y, Deslandes L (2010) Autoacetylation of the Ralstonia solanacearum effector PopP2 targets a lysine residue essential for RRS1-R-mediated immunity in Arabidopsis. PLoS Pathog 6 : e1001202
2009
Dagher R, Briere C, Feve M, Zeniou M, Pigault C, Mazars C, Chneiweiss H, Ranjeva R, Kilhoffer MC, Haiech J (2009) Calcium fingerprints induced by calmodulin interactors in eukaryotic cells. Biochim Biophys Acta 1793 : 1068-1077
Mazars C, Bourque S, Mithofer A, Pugin A, Ranjeva R (2009) Calcium homeostasis in plant cell nuclei. New Phytol 181 : 261-274
Mithofer A, Mazars C, Maffei ME (2009) Probing Spatio-temporal Intracellular Calcium Variations in Plants. Meth Mol Biol 479 : 79-92
Poutrain P, Mazars C, Thiersault M, Rideau M, Pichon O (2009) Two distinct intracellular Ca2+-release components act in opposite ways in the regulation of the auxin-dependent MIA biosynthesis in Catharanthus roseus cells. J Exp Bot 60 : 1387-1398
Vadassery J, Ranf S, Drzewiecki C, Mithofer A, Mazars C, Scheel D, Lee J, Oelmuller R (2009) A cell wall extract from the endophytic fungus Piriformospora indica promotes growth of Arabidopsis seedlings and induces intracellular calcium elevation in roots. Plant J 59 : 193-206
2008
Bernoux M, Timmers T, Jauneau A, Briere C, de Wit PJ, Marco Y, Deslandes L (2008) RD19, an Arabidopsis cysteine protease required for RRS1-R-mediated resistance, is relocalized to the nucleus by the Ralstonia solanacearum PopP2 effector. Plant Cell 20 : 2252-2264
Magnan F, Ranty B, Charpenteau M, Sotta B, Galaud JP, Aldon D (2008) Mutations in AtCML9, a calmodulin-like protein from Arabidopsis thaliana, alter plant responses to abiotic stress and abscisic acid. Plant J 56 : 575-589
Xiong TC, Coursol S, Grat S, Ranjeva R, Mazars C (2008) Sphingolipid metabolites selectively elicit increases in nuclear calcium concentration in cell suspension cultures and in isolated nuclei of tobacco. Cell Calcium 43 : 29-37
2007
Parre E, Ghars MA, Leprince AS, Thiery L, Lefebvre D, Bordenave M, Richard L, Mazars C, Abdelly C, Savoure A (2007) Calcium signaling via phospholipase C is essential for proline accumulation upon ionic but not nonionic hyperosmotic stresses in Arabidopsis. Plant Physiol 144 : 503-512
Ranty B, Aldon D, Galaud JP (2007) Regulation of gene expression by calmodulin in plants. Med Sci (Paris) 23 : 13-14
Walter A, Mazars C, Maitrejean M, Hopke J, Ranjeva R, Boland W, Mithofer A (2007) Structural requirements of jasmonates and synthetic analogues as inducers of Ca2+ signals in the nucleus and the cytosol of plant cells. Angew Chem Int Ed Engl 46 : 4783-4785
PhD students
Amandine Lucchin (2019-2023) Caracterisation fonctionnelle du senseur de calcium AtCML8 dans le développement d’Arabidopsis thaliana.
Julie Mazard (2018-2021) Role de la proteine de signalisation du calcium CML8 dans la reponse d’Arabidopsis thaliana à de multiples stress: implication dans l’immunité contre plusieurs pathogenes et importance de l’auxine.
Ambroise Testard (2014-2017) Rôle de la glycéraldéhyde 3-phosphate déshydrogénase nucléaire lors de mise en place de la mort cellulaire induite par les sphingolipides chez les végétaux.
Manon Perez(2014-2017) Analyse de la contribution de PRR2, un facteur de transcription de type GARP, dans la physiologie des plantes.
Mélanie Ormancey (2016) Signalisation calcique et protéines 14-3-3s dans la mort cellulaire induite par les sphingolipides chez les végétaux.
Xiaoyang Zhu (2016) Contribution de CML8 et CML11, deux “Calmodulin-like proteins” d’Arabidopsis thaliana dans les réponses aux stress.
Mouna Ghorbel (2015) Signalisation calcique et tolérance du blé aux stress abiotiques : rôles des calmodulines dans la modulation d’une MAPK Phosphatase (TMKP1). Thèse en co-tutelle (JP Galaud et M Hanin, Sfax, Tunisie)
Cécilia Cheval (2013) Contribution d’une “Calmodulin-like protein” CML9, et de son partenaire, le facteur de transcription de type GARP PRR2, à la mise en place des réactions de défense chez Arabidopsis thaliana.
Elsa Prigent (2013) Les protéines 14-3-3 et leurs cibles dans la voie de signalisation conduisant à la mort cellulaire programmée en réponse aux sphingolipides : régulation par le calcium.
Louis-Jérôme Leba (2011) Contribution d’AtCML9 aux réactions de défense mises en place en réponse à des stress biotiques chez Arabidopsis thaliana.
Daniel Da Silva (2011) Rôle du compartiment nucléaire dans la signalisation conduisant à la mort cellulaire en réponse aux sphingolipides chez des cellules de tabac BY2.
Yann Aubert (2011) Analyse fonctionnelle de deux protéines de liaison au calcium (AtCML9 et RD20) dans les réponses aux contraintes hydriques chez Arabidopsis thaliana.
Nicolas Amelot (2010) Couplage entre signalisation calcique et modulation du transcriptome en réponse à la cryptogéine chez le tabac. (en co-direction avec l’équipe “Régulation transcriptionnelle”).
Christophe Lachaud (2010) Mort cellulaire induite par les sphingolipides et signalisation calcique chez les végétaux.
Alexandre Perochon (2010) Signalisation calcium chez les plantes : Identification et caractérisation de partenaires de CML9, une protéine réceptrice des signaux calciques, impliquée dans les réponses aux stress de l’environnement chez Arabidopsis thaliana.
Fabienne Magnan (2007) Analyse fonctionnelle d’une protéine de type calmoduline d’Arabidopsis thaliana (AtCML9). Rôle dans les réponses des plantes aux contraintes de l’environnement.
Tou-Cheu Xiong (2005) Le noyau de la cellule végétale est-il autonome en matière de signalisation calcique ?
Elian Perruc (2004) Signalisation calcium chez les végétaux : Caractérisation d’une protéine affine à la calmoduline impliquée dans les réponses aux contraintes de l’environnement.
Frédéric Masclaux (2004) Analyse fonctionnelle d’une famille de protéines membranaires (AtBP-80) chez Arabidopsis thaliana : Recherche, obtention et caractérisation de mutants.
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